Abstract:

Single factor test and orthogonal test methods L9(33) were applied to optimize the technics for extraction and determination of water-soluble polysaccharide in Potentilla anserina L. The results indicated that the optimum technics are as following: solid-liquid ratio 1:30, temperature 100 ℃ and time 3.5 h. Under these conditions, the obtained polysaccharide content is 8.78%. Using improved phenol-sulfuric acid method with glucose as the reference objective, the polysaccharide content in Potentilla anserina L. was also determined by ultraviolet spectrophotometer. The linear range of polysaccharide content is at 10.8～75.6 μg/ml, the regress equation is A=0.0124C+0.0052, R2=0.9987. The determined polysaccharide content is 9.28% and the precision is 0.829% (n=6). The average rate of recovery is 99.33%. In conclusion, this method is simple, easy and reproducible and can be used on the quality control.

Key words: Potentilla anserina L., polysaccharide, extraction, determination