Macroporous Resin Separation and HPLC Method for Determination of Entrapment Efficiency of Sesamin Liposome

doi:10.7506/spkx1002-6630-200902053

FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (2 ): 232-234.doi: 10.7506/spkx1002-6630-200902053

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Macroporous Resin Separation and HPLC Method for Determination of Entrapment Efficiency of Sesamin Liposome

CHEN Zhi-qiang1,2，LIU Yang1

(1. College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin Key Laboratory of Food
Nutrition and Safety, Tianjin 300457, China；2. Tianjin Jianfeng Natural Product Co. Ltd., Tianjin 300457, China)

Received:2008-03-03 Revised:2008-04-30 Online:2009-01-15 Published:2010-12-29
Contact: CHEN Zhi-qiang1,2 E-mail:tedvchen@yahoo.com.cn

Abstract

Abstract:

To evaluate the quality of sesamin liposome, macroporous resin was used to separate the free sesamin and HPLC was used to determine the entrapment efficiency. This method has good linearity in the range of 5 to 200 μg/ml (r=0.9999).The precision RSD is less than 1.47%, the recovery of sesamin with blank liposome is 98.18%, and the average of recovery of blank liposomes through presaturated macroporous resin is 100.35% with an excellent adsorption of free sesamin. This method is simple and reproducible.

Key words: HPLC, macroporous resin, sesamin, entrapment efficiency

CLC Number:

O657.7

CHEN Zhi-qiang1,2，LIU Yang1. Macroporous Resin Separation and HPLC Method for Determination of Entrapment Efficiency of Sesamin Liposome[J]. FOOD SCIENCE, 2009, 30(2 ): 232-234.

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Macroporous Resin Separation and HPLC Method for Determination of Entrapment Efficiency of Sesamin Liposome

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