Preparation of Hydroxysafflor Yellow A Antiserum

doi:10.7506/spkx1002-6630-200903033

FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (3): 150-154.doi: 10.7506/spkx1002-6630-200903033

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Preparation of Hydroxysafflor Yellow A Antiserum

HOU Shi-bin，LU Fang，SU You-hong， ZHANG Fu-chun，LI Jiang-wei*

(Key Laboratory of Biological Resources and Genetic Engineering of Xinjiang, College of Life Science and Technology,
Xinjiang University,Urumqi 830046,China)

Received:2008-01-18 Revised:2008-05-15 Online:2009-02-01 Published:2010-12-29
Contact: HOU Shi-bin E-mail:hsbyzy121@sina.com

Abstract

Abstract:

Objective: To construct artificial immunogen and prepare specific antiserum of hydroxysafflor yellow A (HSYA). Method Immunogen HSYA-BSA and coating antigen HSYA-OVA were prepared by immediate coupling method, and the characterizations of artificial antigen were conducted in color change, ultraviolet and infrared radiation spectrum shift.The polyclonal antiserum was produced from two rabbits immunized with conjugates HSYA-BSA, and then the antiserum was assayed with indirect ELISA method. Results: The ideal coating antigen concentration is 4μg/ml, the available anterium dilution 1:200, and the ideal IgG-HRP dilution is 1:2000. The affinity studies showed that the real detection limit of ELISA for standards is 250 ng/ml with an I50 value of 1.5μg/ml. The linear regression equation is as following: y=－16.213lgx+102.47(r=0.9575), with the linear range from 0.25 to 2μg/ml. Conclusion: The artificial immunogen can be synthesized by immediate coupling method and the polyclonal anti-HSYA antibodies with specificity have been obtained.

Key words: hydroxysafflor yellow A, hapten, antiserum, ELISA

CLC Number:

R927.2

HOU Shi-bin，LU Fang，SU You-hong， ZHANG Fu-chun，LI Jiang-wei*. Preparation of Hydroxysafflor Yellow A Antiserum[J]. FOOD SCIENCE, 2009, 30(3): 150-154.

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Preparation of Hydroxysafflor Yellow A Antiserum

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