Determination of Cyclamate in Preserved Fruits by High-performance Liquid Chromatography Coupled with Pre-column Derivatization

doi:10.7506/spkx1002-6630-200904043

FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (4): 204-206.doi: 10.7506/spkx1002-6630-200904043

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Determination of Cyclamate in Preserved Fruits by High-performance Liquid Chromatography Coupled with Pre-column Derivatization

HE Liang-xing1，LIN Sai-jun2，KE Chun-hui1，GUO Bai-kun1

1. Supervising and Testing Center for Quality and Measurement of Yuhang District of Hangzhou, Hangzhou 311199, China ；
2. Hangzhou Institute of Test and Calibration for Quality and Technology Supervision, Hangzhou 301110, China

Received:2008-02-27 Revised:2008-05-20 Online:2009-02-15 Published:2010-12-29
Contact: GUO Bai-kun E-mail:gbkstarfish@yahoo.com.cn

Abstract

Abstract:

Objective: to establish a simple, accurate and sensitive method for determination of cyclamate in preserved fruits by high-performance liquid chromatography with pre-column derivatization. Method: Through the reaction between cyclamate and sodium hypochlorite under acid medium, N,N-dichloro- cyclohexylamine was determined by UV-HPLC. Results: The detection limit is 0.05 g/kg. The calibration curve is linear in the range of 0.02 to 1.00 mg/ml with a correlation coefficient of 0.9995. The relative standard deviation is 1.56% to 4.49% and the recovery rate is in the range of 91.5% to 98.5%. The determination result has no significant deference from that by the method of GB5009.97 — 2003. Conclusion: The method is sensitivity, simple and stable and has good repetition. It can be used for the determination of cyclamate in preserved fruit.

Key words: cyclamate, high-performance liquid chromatography, pre-column derivatization

CLC Number:

TS207.3

HE Liang-xing1，LIN Sai-jun2，KE Chun-hui1，GUO Bai-kun1. Determination of Cyclamate in Preserved Fruits by High-performance Liquid Chromatography Coupled with Pre-column Derivatization[J]. FOOD SCIENCE, 2009, 30(4): 204-206 https://doi.org/10.7506/spkx1002-6630-200904043

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Determination of Cyclamate in Preserved Fruits by High-performance Liquid Chromatography Coupled with Pre-column Derivatization

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