Molecular Identification of Suspected “Baiyangdu” Strain Based on Internal Transcribed Spacer Sequence Analysis

doi:10.7506/spkx1002-6630-200905032

FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (5): 141-144.doi: 10.7506/spkx1002-6630-200905032

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Molecular Identification of Suspected “Baiyangdu” Strain Based on Internal Transcribed Spacer Sequence Analysis

HOU Jun1，LIN Xiao-min1，JIANG Yun2，NIU Hong-jiang2，LIU Feng2，WANG Chang-rong2，LIU Fan

(1.College of Forestry, Henan University of Science Technology, Luoyang 471003, China；
2.Department of Microbiology, Nankai University,Tianjin 300071, China )

Received:2008-03-28 Revised:2008-09-17 Online:2009-03-01 Published:2010-12-29
Contact: HOU Jun1 E-mail:houjunhoujun@163.com

Abstract

Abstract:

Based on the amplification of the internal transcribed spacer (ITS) sequence with the primers of ITS1 and ITS4, the suspected “Baiyangdu” strain was identified. The results showed that the ITS sequence of 26 strains (15 P. eryngii and 11 P. nebrodensis) registered in GenBank has similarity of 99% to that of“ Baiyangdu” strain. According to the criterion of ITS sequence analysis and characteristics of white pileus and reticulate lamella, the“ Baiyangdu” is identified as a highly affinis strain of P. nebrodensis and P. eryngii.

Key words: “Baiyangdu”strain, ITS, P.nebrodensis, P.eryngii, molecular identification

CLC Number:

HOU Jun1，LIN Xiao-min1，JIANG Yun2，NIU Hong-jiang2，LIU Feng2，WANG Chang-rong2，LIU Fan. Molecular Identification of Suspected “Baiyangdu” Strain Based on Internal Transcribed Spacer Sequence Analysis[J]. FOOD SCIENCE, 2009, 30(5): 141-144.

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Molecular Identification of Suspected “Baiyangdu” Strain Based on Internal Transcribed Spacer Sequence Analysis

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