Abstract:

A new mutant strain GL-5 was obtained from the high-pressure mutant strain G-39 of Rhodotorula glutinis by N+ ion implantation. Its β-carotene yield is up to 17.36mg/L, higher 80.08% than that of the initial strain G-39. Its optimum medium (glucose 40 g/L, peptone 30 g/L, yeast extract 10 g/L, tomato extract 3 ml/L, riboflavin 0.5 ml/L, inoculation amount 50 ml/L, culture broth quantity 50 ml in 250-ml shake flask and initial pH 6.0) for producing β-carotene was obtained by uniform design experiment. Under the above culture conditions, the yield of β-carotene produced by the mutant strain GL-5 reaches 34.21 mg/L, higher 98. 09% than the control. This study suggested that the mutant strain GL-5 is an excellent strain for producingβ-carotene and has bright application prospect in biochemical engineering.

Key words: Rhodotorula glutinis, ion implantation, mutation, β-carotene