FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (7): 148-153.doi: 10.7506/spkx1002-6630-200907034

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Optimization of Fermentation Conditions for Aminopeptidase and X-prolyl-depeptidyl Aminopeptidase (XPDAP) Production by Lactobacillus helveticus

GUO Yu-xing1 PAN Dao-dong1,2,* TIAN Jun-ying1   

  1. (1.National Dairy Products Developing Branch Centre, Nanjing Normal University, Nanjing 210097, China
    2.College of Life Science and Bioengineering, Ningbo University, Ningbo 315211, China)
  • Received:2008-07-04 Revised:2008-11-12 Online:2009-04-01 Published:2010-12-29
  • Contact: PAN Dao-dong1,2,* E-mail:daodongpan@163.com

Abstract:

In this study, the liquid fermentation conditions of Lactobacillus helveticus in MRS medium were optimized for producing aminopeptidase and X-prolyl-depeptidyl aminopeptidase (XPDAP). The effects of initial pH of MRS medium, fermentation temperature and time on aminopeptidase and XPDAP production were evaluated by single-factor test and response surface methodology. The results showed that the highest aminopeptidase and XPDAP yields can be obtained when the initial pH value, fermentation temperature and time are 6.0, 33 ℃ and 16 h, respectively. Also, response surface methodology was proved to be accurate and feasible for condition optimization of enzyme production.

Key words: Lactobacillus helveticus, fermentation, aminopeptidase, X-prolyl-depeptidyl aminopeptidase (XPDAP), response surface methodology

CLC Number: