FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (8): 93-96.doi: 10.7506/spkx1002-6630-200908017

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Application of Response Surface Methodology to Optimization of Polysaccharide Extraction Technology  from Coprinus comatus

YU Jie,CUI Peng-ju,ZHUO Jian-yong,CHEN Mei-zhen   

  1. (Department of Biology, College of Science, Shantou University, Shantou 515063, China)
  • Received:2008-06-11 Revised:2008-11-11 Online:2009-04-15 Published:2010-12-29
  • Contact: CUI Peng-ju, E-mail:cuipengju00@163.com

Abstract:

Effects of extraction temperature, extraction time, ratio of water to material and extraction times on extraction rate of polysaccharide from Coprinus comatu were investigated respectively in single-factor tests, and then other three key factors except extraction times were optimized through response surface methodology with three factors and three levels based on the principle of Box-Behnken design. The results showed that the optimum extraction temperature, time, ratio of water to material and times are 94.5 ℃, .4 h, 15.5:1 and 2, respectively. Under the above conditions, the extraction rate of polysaccharide from Coprinus comatu reaches 6.16%, which is improved by 7.13% through optimization.

Key words: Coprinus comatus polysaccharide, extraction, response surface methodology, Box-Behnken design

CLC Number: