Analysis on Thermal Denaturation of Proteins in Infant Formula Milk Powder Diluted with Water at Different Temperatures Using Matrix-assisted Laser Desorption Ionization Time-of-flight Mass Spectrometry (MALDI-TOF/MS )

doi:10.7506/spkx1002-6630-200909003

FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (9): 21-24.doi: 10.7506/spkx1002-6630-200909003

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Analysis on Thermal Denaturation of Proteins in Infant Formula Milk Powder Diluted with Water at Different Temperatures Using Matrix-assisted Laser Desorption Ionization Time-of-flight Mass Spectrometry (MALDI-TOF/MS )

WANG Zheng-fang1,2 ZOU Ming-Qiang1,* QI Xiao-hua1 ZHANG Zhuo-Yong2

1.Chinese Academy of Inspection and Quarantine, Beijing 100123, China
2. Department of Chemistry, Capital Normal University, Beijing 100037, China

Received:2008-08-01 Revised:2008-12-29 Online:2009-05-01 Published:2010-12-29
Contact: ZOU Ming-Qiang E-mail:mingqiangz@sina.com

Abstract

Abstract:

MALDI-TOF/MS was employed to analyze the relative contents of proteins in infant formula milk powder diluted with water at different temperatures to make clear the change law of protein components of the milk powder. In this experiment, 11 ion peaks in the MALDI-TOF mass spectrum were selected as targets, and 15 parallel tests were performed on each sample. For every target ion, the m/z differed no more than 3 between any two of 15 determinations, the relative error was less than 0.19%, and the RSD was less than 0.19%. The final data were the trimmed values of all the parallel tests. It was indicated that proteins in the milk powder denature gradually with the rising of water temperature, and their denaturation occurrs at a higher degree when water temperature reached 70 ℃. In conclusion, water at 40 ℃ is proper for diluting infant formula milk powder.

Key words: MALDI-TOF/MS, proteins in infant formula milk powder, denaturation

CLC Number:

R151

WANG Zheng-fang1,2 ZOU Ming-Qiang1,* QI Xiao-hua1 ZHANG Zhuo-Yong2. Analysis on Thermal Denaturation of Proteins in Infant Formula Milk Powder Diluted with Water at Different Temperatures Using Matrix-assisted Laser Desorption Ionization Time-of-flight Mass Spectrometry (MALDI-TOF/MS )[J]. FOOD SCIENCE, 2009, 30(9): 21-24.

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Analysis on Thermal Denaturation of Proteins in Infant Formula Milk Powder Diluted with Water at Different Temperatures Using Matrix-assisted Laser Desorption Ionization Time-of-flight Mass Spectrometry (MALDI-TOF/MS )

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