Abstract:

Ethanol injection and rotary film-ultrasonic methods were applied to prepare liposomes with heme iron in this study. Heme iron content and entrapment efficiency in liposomes obtained were measured by spectrophotometry, liposome morphology was observed under optical microscope and transmission electron microscope, and phase changes in cholesterol, lecithin and heme iron were determined by differential scanning calorimetry (DSC). Results showed that the heme iron liposomes prepared by the two methods were basically identical in most indicators. The mass ratio of hemin to cholesterol to phospholipids was in range of (0.1—200): (1—10): (10—100). The liposomes obtained are uniform and big single-chamber particles with an effective diameter of 0.804μm and the maximal entrapment efficiency is 36%.

Key words: heme iron, liposomes, differential scanning calorimetry