HPLC Determination of Hyperoside in Stems and Leaves of Sorbus tianschanica Rupr

doi:10.7506/spkx1002-6630-200912031

FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (12): 154-156.doi: 10.7506/spkx1002-6630-200912031

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HPLC Determination of Hyperoside in Stems and Leaves of Sorbus tianschanica Rupr

YANG Ling-ling，CHANG Jun-min*，YAO Jun

(College of Pharmacy, Xinjiang Medical University, Urumqi 830011, China)

Received:2008-09-09 Revised:2009-01-09 Online:2009-06-15 Published:2010-12-29
Contact: CHANG Jun-min*， E-mail:cjmcjn2471@yahoo.com.cn

Abstract

Abstract:

Hyperoside was extracted from stems and leaves of Sorbus tianschanica Rupr with methanol and then subjected to HPLC analysis. The determination (at 360 nm) was performed on a Shim-pack VP-ODS (150 mm × 4.6 mm, 5 μm) column (25 ℃) using aceyonitrile - water (25:75, V/V) as a mobile phase at the flow rate of 1.0 ml/min. A good linearity was obtained within the range of 0.04 － 0.32 mg and the correlation coefficient was 0.9992. The average recovery for hyperoside was 99.7% and the RSD was 2.6%. The method is simple, rapid, sensitive, precise, reliable and repeatable and can be applied to the quality control of stems and leaves of Sorbus tianschanica Rupr as a kind of Chinese medicinal material.

Key words: Sorbus tianschanica Rupr, hyperoside, HPLC

CLC Number:

R284.1

YANG Ling-ling，CHANG Jun-min*，YAO Jun. HPLC Determination of Hyperoside in Stems and Leaves of Sorbus tianschanica Rupr[J]. FOOD SCIENCE, 2009, 30(12): 154-156.

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HPLC Determination of Hyperoside in Stems and Leaves of Sorbus tianschanica Rupr

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