Simultaneous Determination of Qunolones, Sulfonamides and Tetracyclines Residues in Eel Using Solid Phase Extraction (SPE) and Ultra-performance Liquid Chromatography-Electrospray Tandem Mass Spectrometry (UPLC-MS-MS)

doi:10.7506/spkx1002-6630-200914031

FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (14): 167-170.doi: 10.7506/spkx1002-6630-200914031

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Simultaneous Determination of Qunolones, Sulfonamides and Tetracyclines Residues in Eel Using Solid Phase Extraction (SPE) and Ultra-performance Liquid Chromatography-Electrospray Tandem Mass Spectrometry (UPLC-MS-MS)

LIU Zheng-cai，YANG Fang，YU Kong-jie，LI Yao-ping，LIN Yong-hui，ZHANG Qiong，LIU Su-zhen

Fujian Entry-Exit Inspection and Quarantine Bureau, Fuzhou 350001, China

Received:2008-10-20 Online:2009-07-15 Published:2010-12-29
Contact: LIU Zheng-cai， E-mail:zhengcailiu@yahoo.com.cn

Abstract

Abstract:

A robust and sensitive UPLC-MS-MS method was developed for the simultaneous determination of residues of 15 sulfonamides, 3 fluoroquinolones and 4 tetracylines in eel. The target compounds were extracted with acidic EDTA-Mcllvaine buffer solution, and enriched and cleared up using a hydrophilic-lipophilic balance (HLB) SPE cartridge. After seperation on UPLC BEH C18, the analytes were detected using a tandem mass spectrometry and quantified in multiple reaction monitoring (MRM) mode. At the spike levels of 5, 10 and 20 μg/kg respectively, the average recoveries for all the compounds were 72.4%－97.3%, and the variation coefficients were all under 14.4%. This method is simple, fast, sensitive, and particular for the simulaneous determination of sulfonamides, fluoroquinolones and tetracylines in eel.

Key words: ultra-performance liquid chromatography-tandem mass spectrometry(UPLC-MS-MS), sulfonamides, qunolones, tetracycline, eel

CLC Number:

TS207.53

LIU Zheng-cai，YANG Fang，YU Kong-jie，LI Yao-ping，LIN Yong-hui，ZHANG Qiong，LIU Su-zhen. Simultaneous Determination of Qunolones, Sulfonamides and Tetracyclines Residues in Eel Using Solid Phase Extraction (SPE) and Ultra-performance Liquid Chromatography-Electrospray Tandem Mass Spectrometry (UPLC-MS-MS)[J]. FOOD SCIENCE, 2009, 30(14): 167-170.

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Simultaneous Determination of Qunolones, Sulfonamides and Tetracyclines Residues in Eel Using Solid Phase Extraction (SPE) and Ultra-performance Liquid Chromatography-Electrospray Tandem Mass Spectrometry (UPLC-MS-MS)

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