Abstract:

This study developed a reverse phase HPLC method for determing β-phenylethanol in Chinese rice wine. Betaphenylethanol was separated on a Synergi Hrdro-RP C18 column (4.6 × 250 mm, 4 μm) using a mobile phase composed of CH3OH and H2O (50:50, V/V) at a flow rate of 1 ml/min and detected using a UV detector set at 210 nm. At the spike levels of 5.00, 15.00 and 30.00 mg/L, the average recoveries for β-phenylethanol were between 99.5% and 99.8% with the RSDs of less than 0.6% (n = 3); the limit of detection was 1.0 ng, and the linear range was from 0.5 to 50 mg/L (r = 0.9999). The results of β- phenylethanol determination in four different rice wine samples by this method were almost the same as those determined by gas chromatography method. Therefore, the method developed can be use to determine β-phenylethanol in Chinese rice wine.

Key words: RP-HPLC, Chinese rice wine, β-phenylethanol