Abstract:

Objective: The aim of this study was to evaluate the anti-fatigue effect of Chinese rice wine (CRW) in normal
mice and its influence on immune organ indexes of D-galactose-induced aging mice. Methods: Mice were randomly divided
into four groups: control, low-dose CRW, middle-dose CRW and high-dose CRW groups, which were given by gavage
0.25 mL of distilled water, and 0.15, 0.25 and 0.4 mL of CRW for 15 consecutive days, respectively for exploring the antifatigue
effect of CRW. Then, changes in hepatic glycogen, blood urea nitrogen (BUN) and blood lactic acid (BLA) contents
in mice after forced swimming were measured. A mouse model of sub-acute aging was established by intraperitoneal
injection of D-galactose and the aging mice were respectively given distilled water, aqueous ethanol and CRW once a day
by intragastric administration for 56 days. Finally, the mice were sacrificed, for the measurement of thymus index, spleen
index and body weight gain. Results: CRW could significantly increase hepatic glycogen level (P < 0.01) and enhance postexercise
increase in BLA level (P < 0.05) and the clearance rate of BUN level (P < 0.05). In addition, CRW significantly
inhibited the decrease in thymus (P < 0.01) and spleen (P < 0.05) weights and increased body weight (P < 0.05). Conclusion:
CRW can exert an anti-fatigue effect and enhance immune function in aging mice.

Key words: anti-fatigue effect, immune organ indexes, Chinese rice wine, mice