Abstract:

Various configurations of glucose, fructose and sucrose in honey were determined by nuclear magnetic resonance (NMR) spectroscopy when dissolved in heavy water, their main characteristic peaks in the 1H-NMR spectrum were attributed, and their contents was measured with potussium biphthalate as an internal standard. The RSDs of determination of glucose, fructose and sucrose were 0.33%, 0.17% and 1.34%, respectively, and mean recoveries for them in spiked honey were 96.68%, 98.03% and 95.56%, respectively. This method can be applied to direct determination of small-amount samples with stable and repeatable results and high precision for study of quality standards of honey.

Key words: nuclear magnetic resonance (NMR), honey, glucose, fructose, sucrose, determination