Abstract:

Abstract：This study aimed to develop a new and sensitive immunosorbent biobarcode assay the detection of semicarbazide (SEM), a metabolite of nitrofurazone based on the conjugation of Anti-CPSEM-Au-ssSHDNA. Ascetic monoclonal antibody against CPSEM purified by ammonium sulfate precipitation and ssSHDNA were conjugated to Au nanoparticles with a diameter of 20 nm prepared by controlled reduction of gold chloride with sodium citrate. Then a novel immunosorbent bio-barcode assay, an integration of PCR and idc-ELISA, which can transform enzyme signal into DNA signal, could be developed based on the conjugation of Anti-CPSEM-Au-ssSHDNA. An anti-CPSEM-Au-ssSHDNA complex was successfully prepared, and the immunosorbent bio-barcode DNA detection assay, which could detect 8 pg /ml semicarbazide, was primarily developed.

Key words: furacilin, semicarbazide (SEM), colloidal gold, ssSHDNA