Screening and Structural Analysis of Aptamer Binding to Listeria monocytogenes

doi:10.7506/spkx1002-6630-200919044

FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (19 ): 197-199.doi: 10.7506/spkx1002-6630-200919044

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Screening and Structural Analysis of Aptamer Binding to Listeria monocytogenes

CHEN Min1,2，JIANG Shu-xun1,*，SHAO Bi-ying1，GAO Ru-cheng2，CHEN Wen-bing1，CHEN Bin1，LI Shou-song1，MIAO Ting-yu1

1. Fujian Entry-Exit Inspection and Quarantine Bureau, Fujian Key Laboratory for Technology Research of Inspection and Quarantine,
Fuzhou 350001, China；2. College of Life Sciences, Fujian Normal University, Fuzhou 350108, China

Received:2009-04-23 Online:2009-10-01 Published:2010-12-29
Contact: JIANG Shu-xun

Abstract

Abstract:

Objective: To obtain aptamer that specially binds Listeria monocytogenes. Methods: A custom synthesized 78-mer random ssDNA library was subjected to 10 rounds of selection against Listeria monocytogenes by SELEX method. The selected aptamers were cloned and sequenced. Macaw 2.05 and DNAMAN package were employed to analyze conserved sequences and secondary structure of aptamers, respectively. Results: 6 conserved sequences such as TTTTTTT, TGCTCTT, CGGGGGT, GTTTTT, XTGTT and XTTGT were obtained. Absolutely identical sequences were exhibited in the pairs of NO. 2 and 12, 7 and 9, 158, 159 and 160 aptamer. Secondary structure analysis revealed possible stem-loops and pockets for binding to Listeria monocytogenes. Conclusion: Aptamers with binding capability to Listeria monocytogenes have been obtained by SELEX methods. Their affinities are closely related to the primary structure and the secondary structure.

Key words: SELEX, Listeria monocytogenes, aptamer, primary structure, secondary structure

CLC Number:

Q789

CHEN Min1,2，JIANG Shu-xun1,*，SHAO Bi-ying1，GAO Ru-cheng2，CHEN Wen-bing1，CHEN Bin1，LI Shou-song1，MIAO Ting-yu1. Screening and Structural Analysis of Aptamer Binding to Listeria monocytogenes[J]. FOOD SCIENCE, 2009, 30(19 ): 197-199.

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Screening and Structural Analysis of Aptamer Binding to Listeria monocytogenes

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