Abstract:

Active oligopeptides are the most effective ingredients in deer placenta. The oligopeptides was separated through ultrafiltration from deer placenta. The major factors such as material-liquid ratio, pressure, temperature, flow rate, condensation folds and operation time were investigated to evaluate the effect on separation efficiency. The optimal separation condition was 1.5% of material-liquid ratio, 0.06 MPa of pressure, 30 ℃ of temperature, 2.0 ml/s of flow rate, 2.5 folds for condensation degree and 3 min of operation time. Protein content in separated samples was 56.22% under this optimal condition. The contaminated membrane was cleaned using 0.2% NaOH for 2 h of soaking and 1 h of circulation first, then using HCl for 1 h of soaking and 0.5 h of circulation, and finally using water washing until neutrality. An excellent recovery rate for membrane flux was achieved and WFR value was 0.834.

Key words: deer placenta oligopeptide, ultrafiltration, membrane contamination