Determination of Chlorogenic Acid in Sweet Potato Leaves

doi:10.7506/spkx1002-6630-200924060

FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (24 ): 279-282.doi: 10.7506/spkx1002-6630-200924060

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Determination of Chlorogenic Acid in Sweet Potato Leaves

XIANG Chang-guo1，LI Wen-fang2，XIANG Ning3，HUANG Zhi-lan2，LEI Yong2

1. College of Tourism, Jishou University, Zhangjiajie 427000, China ；
2. College of Resources and Planning Sciences, Jishou University, Zhangjiajie 427000, China；
3. College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China

Received:2009-09-09 Online:2009-12-15 Published:2010-12-29
Contact: XIANG Chang-guo1， E-mail:changguox@yahoo.com.cn

Abstract

Abstract:

Effect of two different developing agents on the separation of chlorogenic acid (CA)was investigated in this paper. The results showed that the composite of ethyl acetate-formic acid-water (7:2.5:2.5, V/V) was suitable for CA separation by TLC. The content of chlorogenic acid determined by TLC, UV and HPLC were 2.67%, 3.07% and 3.31%, respectively, suggesting HPLC was most sensitive. Based on our studies, UV method showed benefits like low cost, simple operation and hence, could be used as generic laboratory tests or normal use. Analysis of stability, recovery and precision of HPLC method indicated that the stability was good and can be maintained above 30 d. The recovery was as high as 101.30% and precision was excellent with the SD less than 1.55%.

Key words: leaves of sweet potatoes, chlorogenic acid, TLC, UV, HPLC

CLC Number:

Q964.81

XIANG Chang-guo1，LI Wen-fang2，XIANG Ning3，HUANG Zhi-lan2，LEI Yong2. Determination of Chlorogenic Acid in Sweet Potato Leaves[J]. FOOD SCIENCE, 2009, 30(24 ): 279-282 https://doi.org/10.7506/spkx1002-6630-200924060

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Determination of Chlorogenic Acid in Sweet Potato Leaves

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