Abstract:

Reversed-phase high performance liquid chromatography was used to purify deer placenta peptide in this paper. Major factors such as addition amount of trifluoroacetic acid, initial concentration of organic phase, flow rate and temperature on separation efficiency were investigated. The optimal purification condition was Hypersil ODS C18 (250 mm × 4.6 mm, 5 μm), 0.05% TFA/CAN as the mobile phase A, 0.05% TFA/water as the mobile phase B, linear gradient elution with 10%－50% mobile phase A and 90%－50% mobile phase B for 30 min, 1.0 ml/min of flow rate at 30 ℃ and detection wavelength at 214 nm.

Key words: reversed-phase high performance liquid chromatogram, deer placenta peptide, purification