Abstract:

In order to investigate exogenous protein expression in oil body system, the fragments of rape oleosin gene promoter (Ycprm) and rape oleosin gene (Ycoil) were obtained through PCR amplification using the designed primers and total DNA from Brassica napus L. VEGF gene was synthesized by overlap extension PCR. An oil body expression plasmid p1390Ycprm-Ycoil- VEGF was constructed by Ycprm, Ycoil and VEGF gene using the reconstruction vector, pCAMBIA1390. This recombinant plasmid was transformed into A. tumefaciens GV3101. A resistant plant, T1, was obtained in 1/2 MS medium containing hygromycin at the concentration of 20 mg/L by screening the seeds (T0) of transformed Arabidopsis plants. The results of PCR, SDS-PAGE and Western blotting showed that the fusion protein with Ycoil gene and VEGF gene were successfully expressed in Arabidopsis thaliana.

Key words: oil body, VEGF, Arabidopsis thaliana, plasmid construction, plant bioreactor