Extraction, Isolation and Protective Effect of Alkaloid from Seabuckthorn Seeds on Injured Cardiomyocytes in Rats

doi:10.7506/spkx1002-6630-201009052

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (9): 234-237.doi: 10.7506/spkx1002-6630-201009052

• Nutrition & Hygiene • Previous Articles Next Articles

Extraction, Isolation and Protective Effect of Alkaloid from Seabuckthorn Seeds on Injured Cardiomyocytes in Rats

HU Chang-ying1，XU De-ping2

1. Department of Food Science and Engineering, Jinan University, Guangzhou 510632, China ；
2. School of Food Science and Technology, Jiangnan University, Wuxi 214122, China

Received:2009-09-21 Revised:2010-01-12 Online:2010-05-01 Published:2010-12-29
Contact: HU Chang-ying E-mail:hucy0000@sina.com

Abstract

Abstract:

Objective: To investigate the protective effect of alkaloid from seabuckthorn seeds on injured cardiomyocytes in rats. Methods: EtOH extract from seabuckthorn seeds was purified by column chromatography and its structure was elucidated by MS and NMR spectroscopic analyses. A model of cardiac ischemic/reperfusion (I/R) in vitro was established using the primary cultured rat cardiomyocytes. The viability of cardiomyocytes was evaluated by MTT assay and the contents of lactate dehydrogenase (LDH) and creatine kinase (CK) were measured to verify the protective effect on injured rat cardiomyocytes by I/R. Results: The structure of water-soluble alkaloid was identified as 5,11-dihydroxyltryptamine, which could promote the survival rate of cardiomyocytes subjected to I/R injury and reduced the release of myocardial enzymes induced by I/R injury. Conclusion: The identified 5,11-dihydroxyltryptamine from seabuckthorn seeds exhibited an excellent protective effect on cultured neonatal rat cardiomyocytes injured by ischemia/reperfusion at the optimal concentration of 150 mg/L.

Key words: seabuckthorn seed, alkaloid, 5,11-dihydroxyltryptamine, cardiomyocyte, ischemia/reperfusion

CLC Number:

R282.7

HU Chang-ying1，XU De-ping2. Extraction, Isolation and Protective Effect of Alkaloid from Seabuckthorn Seeds on Injured Cardiomyocytes in Rats[J]. FOOD SCIENCE, 2010, 31(9): 234-237.

[1]	YANG Zhongmin, SHEN Yihong, HUANG Xianzhi, WANG Zuwen, DING Xiaowen. Improvement Effect of Mulberry Leaf Alkaloids on Abnormal Glucose and Lipid Metabolism and Liver Injury Induced by Oxidative Stress in Mice [J]. FOOD SCIENCE, 2021, 42(7): 156-161.
[2]	WANG Zuwen, SHEN Yihong, HUANG Xianzhi, DING Xiaowen. Effect and Mechanism of Mulberry Leaf Alkaloid on Improving Hepatic Fibrosis in Mice [J]. FOOD SCIENCE, 2021, 42(3): 173-178.
[3]	HAN Haolei, JIANG Changling, WANG Chen, LIU Xin, LU Chengyin, CHEN Hongping. Pyrrolizidine Alkaloids in Tea: A Review of Analytical Methods, Contamination Levels and Health Risk [J]. FOOD SCIENCE, 2021, 42(17): 255-266.
[4]	WANG Zuwen, YANG Zhongmin, HUANG Xianzhi, DING Xiaowen. Improvement Effect of Mulberry Leaf Alkaloids on Hepatic Fibrosis Induced by Carbon Tetrachloride Combined with High-Fat Diet in Mice [J]. FOOD SCIENCE, 2020, 41(7): 146-152.
[5]	YANG Zhongmin, WANG Zuwen, HUANG Xianzhi, DING Xiaowen. Effect and Underlying Mechanism of Mulberry Leaf Alkaloid on Improving D-Galactose-Induced Oxidative Damage in Mice [J]. FOOD SCIENCE, 2020, 41(5): 135-142.
[6]	YANG Zhongmin, SHEN Yihong, WANG Zuwen, HUANG Xianzhi, DING Xiaowen. Mulberry Leaf Alkaloids Improve D-Galactose-Induced Oxidative Damage in the Mouse Kidney [J]. FOOD SCIENCE, 2020, 41(19): 198-203.
[7]	YANG Zhongmin, SHEN Yihong, HUANG Xianzhi, WANG Zuwen, DING Xiaowen. Effect of Crude Alkaloids from Mulberry Leaves on Improving D-Galactose-Induced Oxidative Protein Damage in Mice [J]. FOOD SCIENCE, 2020, 41(17): 182-187.
[8]	WANG Zuwen, SHEN Yihong, HUANG Xianzhi, DING Xiaowen. Effects of Mulberry Leaf Alkaloids on Antioxidant Capacity and Inflammatory Cytokine Levels in Mice with Hepatic Fibrosis [J]. FOOD SCIENCE, 2020, 41(17): 202-207.
[9]	WANG Zuwen, YANG Zhongmin, YANG Min, HUANG Xianzhi, DING Xiaowen. Effect and Mechanism of Mulberry Leaf Alkaloids on the Improvement of High-Fat Diet Induced Liver Injury in Mice [J]. FOOD SCIENCE, 2019, 40(19): 210-216.
[10]	TANG Qin, SUN Weijiang, CHEN Zhidan, SHEN Shiyu, ZHOU Zhe, CHEN Jiajia, CHEN Cuicui. Determination and Analysis of Bitter and Astringent Substances in Youxi Bitter Tea Resources [J]. FOOD SCIENCE, 2019, 40(18): 242-247.
[11]	ZHANG Lang, HAO Ji, HUANG Xu, WU Chaoqun, FENG Tianhui, SHU Guangwen. Total Alkaloids from the Seed Embryo of Nelumbo nucifera Ameliorate Inflammation in the Livers of Mice with Lipopolysaccharide/D-Galactosamine-Induced Acute Liver Failure [J]. FOOD SCIENCE, 2018, 39(5): 220-225.
[12]	ZHAO Xiuling, DANG Yali. Recent Advances in Chemical Components, Extraction and Pharmacological Effects of Nelumbinis Plumula [J]. FOOD SCIENCE, 2018, 39(23): 329-336.
[13]	FAN Sufang, MA Junmei, LIU Zhuo, YU Jing, ZHAI Hongwen, LI Qiang, ZHANG Yan. Determination of 15 Toxic Alkaloids in Food Samples by High Performance Liquid Chromatography-Tandem Mass Spectrometry [J]. FOOD SCIENCE, 2018, 39(22): 283-288.
[14]	HAO Mengmeng, CUI Hanzhao, HAN Aizhi, YANG Ling. Extraction of Active Substances from Leaves of Morus nigra L. and Evaluation of Their Inhibitory Effect on α-Glycosidase [J]. FOOD SCIENCE, 2018, 39(19): 19-23.
[15]	LIU Liyuan, CHEN Min, ZHAO Haitian, YAO Lei. Recent Advances in Understanding the Radioprotective Effect of Natural Products [J]. FOOD SCIENCE, 2018, 39(17): 269-274.

Extraction, Isolation and Protective Effect of Alkaloid from Seabuckthorn Seeds on Injured Cardiomyocytes in Rats

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments