FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (12): 93-96.doi: 10.7506/spkx1002-6630-201012021

• Technology Application • Previous Articles     Next Articles

Separation of Formononetin from Millettia nitita var. hirsutissima Using High-Speed Counter-current Chromatography

RUAN Zheng1,2，WU Chang-qiang1,2，YIN Yu-long1,2,3，DENG Ze-yuan1,2,*   

  1. (1. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China；
    2. College of Life Science and Food Engineering, Nanchang University, Nanchang 330031, China；
    3. Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China)
  • Received:2009-10-11 Online:2010-06-15 Published:2010-12-29
  • Contact: DENG Ze-yuan1,2,* E-mail:dengzy28@yahoo.com.cn

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Abstract:

Purpose: To develop the experimental system for the separation of formononetin from Millettia nitita var. hirsutissima by high-speed counter-cunent chromatography (HSCCC). Methods: The optimum solvent composition for HSCCC separation was determined based on partition coefficients and phase separation times for various fractions of the Millettia nitita var. hirsutissima. The purity of the formononetin fraction from HSCCC separation was measured by HPLC. Results: A mixture of n-hexane, ethylacetate, methanol and water (4:5:4:5, V/V) was the selected solvent system, the upper phase and the lower phase separated from which provided mobile phase and stationary phase, respectively. The HSCCC separation of formononetin was carried out under the following conditions: flow rate 2.0 mL/min, rotation speed 800 r/min and 26 ℃. The yield of formononetin purified from the ether extract from Millettia nitita var. hirsutissima was 16.1%, with 96.3% purity. Conclusion: The developed solvent system can provide a reliable separation of formononetin. The HSCCC method is applicable for the rapid and efficient separation of formononetin.

Key words: Millettia nitita var. hirsutissima, HSCCC, formononetin, HPLC

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