Abstract:

Objective: To achieve full utilization of the Prunus sibirica L. resource, the fruit flesh of the plant was extracted to obtain water soluble polysaccharides (WSP). Methods: Single factor and orthogonal array design methods were used for providing experimental data for the response surface optimization of WSP yield, in which a mathematical model predicting WSP yield as a function of extraction conditions was established. Results: Extraction temperature was the most import factor affecting WSP yield, followed by length of extraction time and material-to-water ratio (mg/mL). The optimum levels of these 3 extraction conditions were found to be: material-to-water ratio 1:26 and extraction temperature 94 ℃ for an extraction duration of 2.8 h and the optimized extraction provided a WSP yield of 3.44%. Conclusion: The close agreement between the observed and predicted values of WSP yield demonstrates high reliability of the established model.

Key words: Prunus sibirica L., polysaccharides, optimization of extraction technology