Abstract:

A two-step steeping method was used to produce corn starch. In the second step, a natrium sisulfurosum suspension of Saccharomyces cerevisiae was used to steep corn. Through single factor investigations, the optimum conditions for the first step were determined to be: natrium sisulfurosum concentration 0.15 g/mL, lactic acid concentration 0.3 g/mL, steeping temperature 50 ℃ and steeping time 10 h, and those for the second step were as follows: steeping temperature 40 ℃, steeping time 24 h, natrium sisulfurosum concentration 0.1 g/mL and inoculum size 5% (V/V). This steeping method could largely shorten production cycle and lower the cost of starch production.

Key words: natrium bisulfurosum, two-step steeping, Saccharomyces cerevisiae, starch production