Isolation and Identification of a Lactobacillus plantarum Strain and Sequence Analysis of Its Cryptic Plasmid

doi:10.7506/spkx1002-6630-201017053

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (17): 236-239.doi: 10.7506/spkx1002-6630-201017053

• Bioengineering • Previous Articles Next Articles

Isolation and Identification of a Lactobacillus plantarum Strain and Sequence Analysis of Its Cryptic Plasmid

DU Li-hui1，HUO Gui-cheng2,*，JU Xing-rong1，LIU Fang3

1. College of Food Science and Engineering, Nanjing University of Finance and Economics, Nanjing 210003, China；2. Key
Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030, China ；3. Institute of
Agricultural Products Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China

Received:2010-01-05 Online:2010-09-15 Published:2010-12-29
Contact: HUO Gui-cheng E-mail:xingrongju@163.com

Abstract

Abstract:

A strain isolated from traditionally fermented dairy product was identified as Lactobacillus plantarum based on API 50 CH bacteria identification system and 16S rRNA sequence analysis. Plasmid profile indicated that this strain harbored several plasmids. A small plasmid designated as pLD1 was subjected to sequence analysis. This small plasmid had 2112 bp and G+C content of 37.8%. Meanwhile, a sequence with 17 bp was repeated 13 times in this small plasmid. BLAST results showed that pLD1 plasmid had more than 95% similarity with pLP2000 and that there was a difference in replication protein among this plasmid and others. The accession number of this small plasmid is NC_012220 and it is useful for the construction of genetically engineered vectors.

Key words: Lactobacillus plantarum, cryptic plasmid, sequence analysis, replication protein

CLC Number:

Q93.331

DU Li-hui1，HUO Gui-cheng2,*，JU Xing-rong1，LIU Fang3. Isolation and Identification of a Lactobacillus plantarum Strain and Sequence Analysis of Its Cryptic Plasmid[J]. FOOD SCIENCE, 2010, 31(17): 236-239.

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Isolation and Identification of a Lactobacillus plantarum Strain and Sequence Analysis of Its Cryptic Plasmid

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