FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (18): 95-98.doi: 10.7506/spkx1002-6630-201018022

• Processing Technology • Previous Articles     Next Articles

Preparation and Chromatographic Evaluation of Molecularly Imprinted Polymer Microspheres for Chloramphenicol

SHI Xi-zhi1，YANG Hua2，SUN Ai-li1，LI De-xiang1，CHEN Jiong1   

  1. 1. Faculty of Life Science and Biotechnology, Ningbo University, Ningbo 315211, China；
    2. College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo 315100, China
  • Received:2010-05-19 Revised:2010-08-24 Online:2010-09-25 Published:2010-12-29

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Abstract:

A uniformly sized molecularly imprinted polymer microspheres (MIPMs) for chloramphenicol (CAP) has been prepared by a two-step swelling and polymerization method using 2-(Diethylamino) ethyl methacrylate (DEAEM), ethylene glycol dimethacrylate (EGDMA) and polystyrene microspheres as the functional monomer, cross-linker and seed particles, respectively. The porous surface of MIPMs could be clearly observed under scanning electron microscope (SEM). Furthermore, the MIPMs had a larger pore volume and pore diameter measured using BELSORP-miniII than the non-imprinted polymer microspheres (NIPMs) did. The selectivity of MIPMs was assessed by chromatographic method, and CAP, thiamphenicol (TAP) and florfenicol (FF) could reach a stronger separation on the MIPMs with a MeCN/water/H3PO4 mixture (30:70:0.1,V/V) as the mobile phase.

Key words: chloramphenicol, molecularly imprinted polymer microspheres, thiamphenicol, florfenicol

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