Abstract:

Eight common proteases of the same concentrations were co-immobilized pairwise into sodium alginate beads at a volume ratio of 1:1 in order to provide co-immobilized enzymes for hydrolyzing peanut meal to obtain polysaccharides. The hydrolysis process of peanut meal based on the use of co-immobilized enzymes was optimized. Combination of acidic protease and papain gave a maximum polysaccharide yield among six combinations and the optimal conditions for hydrolyzing peanut meal with this combination were as follows: enzyme concentration for immobilization 10%; solid/liquid ratio 1:20; pH 5.0; and hydrolysis duration 5 h. A polysaccharide yield of 10.88% was achieved under these conditions. After repeated use for 7 times, the co-immobilized enzymes still had an activity exceeding 50% of the original activity.

Key words: co-immobilized enzymes, extraction, peanut, polysaccharides