Morphological Study on Hela Cells Apoptosis Induced by Lycium barbarum Polysaccharides

doi:10.7506/spkx1002-6630-201019072

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (19): 329-334.doi: 10.7506/spkx1002-6630-201019072

• Nutrition & Hygiene • Previous Articles Next Articles

Morphological Study on Hela Cells Apoptosis Induced by Lycium barbarum Polysaccharides

ZHU Cai-ping1，ZHANG Sheng-hua2，XIAO Jun-xia2

1. College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi’an 710062, China ；
2. College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China)

Received:2010-02-05 Revised:2010-07-16 Online:2010-10-15 Published:2010-12-29
Contact: ZHU Cai-ping1 E-mail:zcaiping@snnu.edu.cn

Abstract

Abstract:

Lycium barbarum polysaccharides (LBP), isolated with boiling water from Lycium barbarum fruits, a famous Chinese medicinal herb, is one of the most important functional constituents in Lycium barbarum. In this study, the effect of LBP at the range of 3.125 to 200 mg/L on the proliferation of Hela cells was measured. After Hela cells were treated with LBP, typical apoptotic morphological changes were observed by fluorescence microscope, transmission electron microscope (TEM) and laser scanning confocal microscope (LSCM). Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay also confirmed that LBP-treated Hela cells showed apoptotic features. The results suggest that LBP is a potential antitumor compound.

Key words: Lycium barbarum polysaccharides, human cervical carcinoma Hela cells, apoptosis, morphology

CLC Number:

R151.1

ZHU Cai-ping1，ZHANG Sheng-hua2，XIAO Jun-xia2. Morphological Study on Hela Cells Apoptosis Induced by Lycium barbarum Polysaccharides[J]. FOOD SCIENCE, 2010, 31(19): 329-334.

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Morphological Study on Hela Cells Apoptosis Induced by Lycium barbarum Polysaccharides

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