Time-resolved Fluoroimmunoassay of Clenbuterol Hydrochloride using Europium Labeled Antigen

doi:10.7506/spkx1002-6630-201020063

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (20): 307-310.doi: 10.7506/spkx1002-6630-201020063

• Analysis & Detection • Previous Articles Next Articles

Time-resolved Fluoroimmunoassay of Clenbuterol Hydrochloride using Europium Labeled Antigen

FAN Xiao-bo1,2，YANG Jin-yi2，GAO Xiu-jie2，WU Ying-song2，SUN Yuan-ming2，LEI Hong-tao2,*

1. College of Food Science and Engineering, Northwest A & F University, Yangling 712100, China；
2. Key Laboratory of Food Quality and Safety of Guangdong Province, College of Food Science,
South China Agricultural University, Guangzhou 510642, China

Received:2009-12-28 Revised:2010-06-26 Online:2010-10-25 Published:2010-12-29
Contact: LEI Hong-tao2 E-mail:hongtao@scau.edu.cn

Abstract

Abstract:

A convenient, rapid and sensitive method has been presented for the determination of clenbuterol hydrochloride (CBL) using the direct competitive time-resolved fluoroimmunoassay (TRFIA). In the direct competitive assay, the anti-CBL monoclonal antibody was bound on the surface of a 96-cell microplate, and samples containing CBL or the standards competed for the antibody binding sites with Eu3+ labeled antigen in the wells of microplate. The determinations of CBL in porcine urine and tissue samples showed that the limit of detection was 0.02μg/L, with spike recoveries ranging from 91% to 101%. The cross-reaction rates with other six drugs were less than 1%. The proposed TRFIA method is sensitive and specific, and can be used for the determination of CBL residue in food samples.

Key words: cenbuterol hydrochloride (CBL), time-resolved fluoroimmunoassay (TRFIA), antigen, detection

CLC Number:

X792

FAN Xiao-bo1,2，YANG Jin-yi2，GAO Xiu-jie2，WU Ying-song2，SUN Yuan-ming2，LEI Hong-tao2,*. Time-resolved Fluoroimmunoassay of Clenbuterol Hydrochloride using Europium Labeled Antigen[J]. FOOD SCIENCE, 2010, 31(20): 307-310.

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Time-resolved Fluoroimmunoassay of Clenbuterol Hydrochloride using Europium Labeled Antigen

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