FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (24): 177-181.doi: 10.7506/spkx1002-6630-201024038

• Processing Technology • Previous Articles     Next Articles

Optimization of Two-phase Solvent System Extraction Processing for Isoflavone from Flos puerariae by Response Surface Methodology

FENG Wei-xi1，ZHANG Yong-dan2，HAN Ting2，LIU Ying2，CHENG Gang3，HUANG Wen2,*   

  1. 1. Lianyungang TCM Branch of Jiangsu Union Technical Institute, Lianyungang 222006, China；
    2. College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China；
    3. College of Life Science, South Central University for Nationalities, Wuhan 430078, China
  • Received:2010-06-09 Online:2010-12-25 Published:2010-12-29
  • Contact: HUANG Wen E-mail:huangwen@mail.hzau.edu.cn

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Abstract:

Flos puerariae was used as raw material to extract isoflavone using n-buatnol/water two-phase system as solvents. Based on single factor experiments, the optimal extraction processing was explored by central design composite experiments combined with response surface methodology. Results showed that the optimal extraction processing parameters were extraction time of 1.3 h, extraction temperature of 70 ℃, n-buatnol-water ratio of 1:1 (V/V) and material-liquid ratio of 1:55 (g/mL). Under these optimal conditions, the extraction rate of isoflavone was 96.24%, which was very close to predicted value. Therefore, it is feasible to optimize the extraction processing of isoflavone from Flos puerariae by response surface methodology.

Key words: two-phase system, Flos puerariae, isoflavone, response surface methodology, extraction

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