Screening and Identification of a Strain Capable of Producing Acid Phytase and Optimization of  Its Fermentation Conditions

doi:10.7506/spkx1002-6630-201105049

FOOD SCIENCE ›› 2011, Vol. 32 ›› Issue (5 ): 228-233.doi: 10.7506/spkx1002-6630-201105049

• Bioengineering • Previous Articles Next Articles

Screening and Identification of a Strain Capable of Producing Acid Phytase and Optimization of Its Fermentation Conditions

LI Wen，WANG Tao

College of Food (Biological) Engineering, Xuzhou Institute of Technology, Xuzhou 221008, China

Received:2010-06-22 Revised:2011-02-12 Online:2011-03-15 Published:2011-03-03
Contact: LI Wen， E-mail:wenlisony@163.com

Abstract

Abstract: A strain with higher acid phytase activity isolated from soil was identified as Aspergillus niger. The optimal fermentation conditions for improved production of phytase by the strain were incubation in a medium composed of 3 g/100mL wheat bran and 0.5 g/100mL (NH4)2SO4, at an initial pH of 5.5 for 84 h. Under the optimal fermentation conditions, the phytase activity was up to 12.06 U/mL. The optimal reaction pH and temperature of this enzyme were 5 and 45 ℃, respectively. The relative enzyme activity remained to be 60% after heating treatment at 55 ℃ for 30 min. In addition, the enzyme was stable in acidic and neutral media. The phytase activity was inhibited by Ca2+, Fe2+, Na+, K+, Zn2+ and Cu2+, while activated by Mg2+ and Ba2+.

Key words: acid phytase, screening, fermentation, enzyme property

CLC Number:

Q93.331

LI Wen，WANG Tao. Screening and Identification of a Strain Capable of Producing Acid Phytase and Optimization of Its Fermentation Conditions[J]. FOOD SCIENCE, 2011, 32(5 ): 228-233.

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Screening and Identification of a Strain Capable of Producing Acid Phytase and Optimization of Its Fermentation Conditions

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