Synthesis and Identification of Oxytetracycline and Ractopamine Antigens

doi:10.7506/spkx1002-6630-201109038

Abstract

Abstract: In order to explore the preparation of antigen for ractopamine (RAC) and oxytetracycline (OTC), RAC and OTC were conjugated with the carrier protein BSA by ethyl carbodiimide hydrochloride (EDC) method and mixed anhydride method to achieve RAC-BSA and OTC-BSA, respectively. Both OTC-BSA and RAC-BSA were identified with UV-visible spectroscopy to reveal the successful coupling with a molar coupling ratio of 5:1 and 7:1, respectively. Polyclonal antibodies were prepared by immunizing New Zealand white rabbits with RAC-BSA and OTC-BSA. The obtained polyclonal antibodies can be used to detect RAC and OTC.

Key words: oxytetracycline, ractopamine, coupling, antigen

CLC Number:

TS202.3

SONG Shan-shan，PENG Chi-fang，KUANG Hua，YAN Wen-jing，SUN Meng-meng，XU Chuan-lai. Synthesis and Identification of Oxytetracycline and Ractopamine Antigens[J]. FOOD SCIENCE, 2011, 32(9): 167-169.

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Synthesis and Identification of Oxytetracycline and Ractopamine Antigens

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