Antibacterial Mechanisms of Fructus mume Extract against Listeria innocua

doi:10.7506/spkx1002-6630-201115021

Abstract

Abstract: The antibacterial mechanisms of ethanol extract from Fructus mume (prepared by reflux extraction with 95% ethanol and subsequent rotary evaporation until a concentration of 1 g/mL, namely raw material weight/condensed extract volume ratio = 1:1) against Listeria innocua were assayed by flow cytometry. The minimum inhibitory concentration (MIC) of the extract against L. innocua was 0.625 mg/mL. In the presence of the extract at different concentrations such as 1, 2 and 4 MICs, the changes of forward scatter (FSC) light, DNA content, membrane potential, cell cycle, Ca2+ and Annexin V-FITC/PI double staining of L. innocua were analyzed by flow cytometry. The results showed that the extract could inhibit L. innocua by destroying cell membrane system and blocking DNA synthesis.

Key words: flow cytometry, Fructus mume, L. innocua, antibacterial mechanism

CLC Number:

TS201.3

GENG Fei，WANG Wei，ZHOU Tao. Antibacterial Mechanisms of Fructus mume Extract against Listeria innocua[J]. FOOD SCIENCE, 2011, 32(15): 88-93.

[1]	GUO Juan, ZHANG Jin, WANG Jiamin, ZHU Quanshan. Natural Antibacterial Agents and Their Application in Food Packaging: A Review [J]. FOOD SCIENCE, 2021, 42(9): 336-346.
[2]	SUN Yue, LIU Jiayi, CHEN Lu, DU Hong, BAI Fengling, LÜ Xinran, ZHANG Defu, GUO Xiaohua, LI Jianrong. Screening of Lactic Acid Bacteria for Antagonistic Activity against Drug-Resistant Escherichia coli and Underlying Mechanism [J]. FOOD SCIENCE, 2021, 42(2): 121-127.
[3]	WANG Ming, ZHANG Jiatao, ZHOU Bin, DING Jie, XU Zhaomeng, SUN Tong. Antibacterial Mechanism of Eugenol-Containing Composite Preservatives against Shewanella putrefaciens [J]. FOOD SCIENCE, 2021, 42(13): 10-16.
[4]	SHI Xu, LIU Ying, SHI Haisu, WU Junrui, WU Rina, NIU Xueqing, LUO Xue, YUE Xiqing. Directed Evolution of Lactobacillus plantarum and Propionibacterium acnes for Increased Production of Conjugated Linoleic Acid [J]. FOOD SCIENCE, 2021, 42(12): 99-106.
[5]	FU Yun, ZHAO Mouming, PANG Yiyang, LIU Xiaoling. Comparative Study on the Anti-Staphylococcus aureus Mechanism of SP-AP-1 and Iturin A Derived from Fermentation of Spirulina Residue by Bacillus subtilis [J]. FOOD SCIENCE, 2021, 42(1): 108-114.
[6]	NING Yawei, SU Dan, FU Yunan, HAN Panpan, WANG Zhixin, JIA Yingmin. Antibacterial Mechanism of Antimicrobial Peptide Brevilaterin Combined with ε-Polylysine against Staphylococcus aureus [J]. FOOD SCIENCE, 2020, 41(5): 15-22.
[7]	HU Yingying, LI Qixuan, LIU Haotian, KONG Baohua, CHEN Qian. Bactericidal Mechanism of Nano-Titanium Dioxide Photocatalysis Technology and Its Application in Food Packaging: A Literature Review [J]. FOOD SCIENCE, 2020, 41(3): 232-238.
[8]	NING Yawei, FU Yunan, HE Jianzhuo, SU Dan, HOU Linlin, WANG Zhixin, JIA Yingmin. Synergistic Antibacterial Mechanism of Phenyllactic Acid Combined with Acetic Acid against Listeria monocytogenes [J]. FOOD SCIENCE, 2020, 41(23): 70-76.
[9]	NING Yawei, SU Dan, FU Yunan, LIU Yangliu, WANG Zhixin, JIA Yingmin. Antibacterial Mechanism of Antimicrobial Peptide Brevilaterin Combined with Citric Acid against Escherichia coli [J]. FOOD SCIENCE, 2020, 41(19): 31-37.
[10]	GE Da’e, WEI Zhaohui, Tursunay · TURGUN, PAN Yue, WANG Fan, TAO Ningping, ZHOU Jianzhong, LIU Xiaoli. Inhibitory Effect of Eugenol on Alternaria sp. Isolated from Blueberry [J]. FOOD SCIENCE, 2020, 41(19): 68-73.
[11]	BAO Yihong, ZHANG Junshun, FU Qun, ZHANG Haiting. Antibacterial Activity and Mechanism of Berberine from the Fruit of Berberis poiretii [J]. FOOD SCIENCE, 2020, 41(17): 29-34.
[12]	SHU Huizhen, TANG Zhiling, HAN Wei, CHEN Haiming, CHEN Weijun, HU Yueying, CHEN Wenxue. Antibacterial Activity and Mechanism of Caryophyllene against Brochothrix thermosphacta [J]. FOOD SCIENCE, 2020, 41(15): 31-38.
[13]	WU Kegang, ZHAO Xinxin, DUAN Xuejuan, CHAI Xianghua, YU Hongpeng, LIU Xiaoli, FAN Yuting. Antibacterial Activity and Mechanism of Action of Vapor-Phase Linalool [J]. FOOD SCIENCE, 2020, 41(1): 61-67.
[14]	NING Yawei, YANG Kun, HE Jianzhuo, ZHANG Yan, LI Qiang, WANG Zhixin, JIA Yingmin. Sucrose Laurate Exerts Antibacterial Activity against Staphylococcus aureus by Destroying the Cell Membrane [J]. FOOD SCIENCE, 2019, 40(5): 38-44.
[15]	ZHOU Qi, YUAN Kang, LIU Fang, DU Lihui. Antimicrobial Activity and Mechanism of Carvacrol against Enterobacter cloacae [J]. FOOD SCIENCE, 2019, 40(13): 22-27.

Antibacterial Mechanisms of Fructus mume Extract against Listeria innocua

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments