Abstract: Objective: To explore the anti-oxidative stress effect of water-soluble soybean dietary fiber (WSSDF) prepared by ultrasound-assisted extraction (UAE) or alkali extraction (AE) on the kidneys of hyperdipemic mice. Methods: Kuming male mice were randomly divided into 8 groups. The mouse groups were fed a normal diet or a high fat diet alone or supplemented with WSSDF (400, 200mg/(kg ·d) and 100 mg/(kg ·d)) derived from UAE and WSSDF (400, 200 mg/(kg ·d) and 100 mg/(kg ·d)) derived from AE, respectively. After 15 consecutive days of intrasgastric SDF administration, serum TC, TG, HDL-C and LDL-C and kidney MDA, H2O2, GSH, GST, SOD and CAT were determined. Results: Compared with the model group, both WSSDFs derived from UAE and AE could decrease the contents of GST, MDA and H2O2, increase the activities of GSH, SOD and CAT in the kidneys of hyperdipemic mice. Conclusion: WSSDF can improve antioxidant stress in kidney of hyperdipemic mice in a dose-dependent manner and UAE results in a slight increase of the activity of WSSDF when compared with AE.

Key words: soybean dietary fiber, ultrasound-assisted extraction, alkali extraction, hyperdipemic, oxidative stress