FOOD SCIENCE ›› 2011, Vol. 32 ›› Issue (22): 75-80.doi: 10.7506/spkx1002-6630-201122016

• Processing Technology • Previous Articles     Next Articles

Optimization of Enzymatic Preparation of Royal Jelly-derived Peptides with High Angiotensin ⅠConverting Enzyme (ACE) Inhibitory Activity

CHEN Jing,LIU Chao,TU Shi,XU Li-man,LIU Rui*   

  1. (Wuhan Research Center on Quality Control Engineering of Bee Products, College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China)
  • Online:2011-11-25 Published:2011-11-11

Abstract: Amano protease P was selected as the most suitable enzyme out of 5 proteases for preparing royal jelly-derived peptides with high angiotensinⅠconverting enzyme (ACE) inhibitory activity. Using one-factor-at-a-time method in combination with quadratic rotary orthogonal combination design, the optimal conditions for the hydrolysis of royal jelly by the selected enzyme for further improving ACE inhibitory rate of hydrolysates were 196 min, 0.0653 g/mL substrate concentration and 1503 U/g enzyme amount, resulting in an experimental ACE inhibitory rate of 79.75%, representing 98.08% of the theoretical value. Hydrolysis time and substrate concentration had extremely significant effect on ACE inhibitory rate of hydrolysates (P<0.01) and enzyme amount had significant effect (P<0.05). In conclusion, royal jelly almost has no ACE inhibitory activity, while royal jelly-derived peptides resulting from Amano protease P-catalyzed hydrolysis have powerful ACE inhibitory activity.

Key words: royal jelly, angiotensinⅠconverting enzyme (ACE)-inhibitory rate, protease, hhydrolysis, response surface analysis

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