Primer Design and PCR Detection of Potato and Tomato Endogenous Genes

doi:10.7506/spkx1002-6630-201122039

Abstract

Abstract: The specific fragments of potato pyrophosphorylase gene (UGPase, U20345) and tomato polygalacturonase-2a gene (PG-2a, X04583.1) were identified by BLAST analysis and multiple sequence alignment. Two pairs of primer were subsequently designed and demonstrated high specificity by PCR. A new method was developed to detect potato and tomato product simultaneously by duplex PCR, and 1.25 ng of mixed potato and tomato DNA was successfully detected. With high specificity and sensitivity, this method is applicable for the detection of potato and tomato products by entry-exit inspection and quarantine departments.

Key words: potato, tomato, endogenous gene primer, UGPase, PG-2a, duplex PCR (polymerase chain reaction)

CLC Number:

CAIHua，LUANFeng-xia，GUANXue-jia，ZHUWei，BAIYue. Primer Design and PCR Detection of Potato and Tomato Endogenous Genes[J]. FOOD SCIENCE, 2011, 32(22): 192-195.

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Primer Design and PCR Detection of Potato and Tomato Endogenous Genes

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