An Investigation into the Self-assembly Behavior of Collagens from Grass Carp Scale

doi:10.7506/spkx1002-6630-201123002

Abstract

Abstract: In this study, acid-soluble collagen (ASC) and pepsin-soluble collagen (PSC) were extracted from grass carp scale and molecularly characterized. Meanwhile, a preliminary investigation into their self-assembly behavior in vitro was carried out along with a comparison with porcine skin collagen(PC). ASC, PSC and porcine skin collagen were all type Ⅰcollagens and showed a high similarity in molecular weight and secondary structure but a difference in amino acid composition. The results of turbidity tests indicated that all the collagens exhibited behavior in vitro under the appropriate temperature and pH. The process of self-assembly consisted of lag, rapid rise and plateau phases and their occurrence times were affected by medium pH. Under the same conditions, the self-assembly degree of porcine skin collagen was highest, followed by PSC and ASC. Environmental temperature, collagen concentration and thermal denaturation temperature had different effects on the self-assembly behavior in vitro of PSC.

Key words: grass carp, fish scale, collagen, self-assembly

CLC Number:

TS254.1

LIANGYan-ping，WANGYan，WANGHai-bo，ZHANGHan-jun，ZHOUJian，LIULiang-zhong. An Investigation into the Self-assembly Behavior of Collagens from Grass Carp Scale[J]. FOOD SCIENCE, 2011, 32(23): 15-20.

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An Investigation into the Self-assembly Behavior of Collagens from Grass Carp Scale

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