Analysis of Phenols, Flavonoids and Oxygen Radical Absorbance Capacity (ORAC) of Three Varieties of Adlay

doi:10.7506/spkx1002-6630-201201014

Abstract

Abstract:

In order to explore the phytochemical profile of three adlay varieties, the levels of total, free and bound phenols and flavonoids were determined using a microplate reader. Meanwhile, the oxygen radical absorbance capacity (ORAC) was measured as an indicator for total antioxidant capacity using a multifunctional fluorescence microplate reader. The contents of free ((45.19 ± 0.91) mg of gallic acid equivalent/100 g of dried samples) and bound ((30.86 ± 1.18) mg of gallic acid equivalent/100 g samples) phenols in Longyi I were significantly higher than those in Liaoning V and Guizhou Heigu (P ＜ 0.05). For all these adlay varieties, bound phenols averagely accounted for 45.28% of total phenols. The content of free flavonoids in Liaoning V showed a significant increase as compared with Longyi I and Guizhou Heigu, and the content of bound flavonoids in Longyi I was higher than that of Liaoning V and Guizhou Heigu. The highest content of total flavonoids was found in Liaoning V. The adlay varieties were ranked in decreasing order of ORAC as follows: Longyi I ((668.0 ± 32.7) mg of Trolox equivalent/100 g of dried samples), Liaoning V and Guizhou Heigu. The average percentage of bound ORAC in total ORAC was 48.08%. A correlation coefficient (r2) of 0.933 was observed for the significant correlation (P ＜ 0.05) between the total phenol content and total antioxidant capacity of each adlay variety.

Key words: adlay, phenols, flavonoids, oxygen radical absorbance capacity(ORAC)

CLC Number:

TS201.1

WANG Li-feng，HE Rong，YUAN Jian，JU Xing-rong. Analysis of Phenols, Flavonoids and Oxygen Radical Absorbance Capacity (ORAC) of Three Varieties of Adlay[J]. FOOD SCIENCE, 2012, 33(1): 72-76.

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