Purification and Identification of Proline-rich Polypeptides from Bovine Colostrum

doi:10.7506/spkx1002-6630-201201015

FOOD SCIENCE ›› 2012, Vol. 33 ›› Issue (1): 77-80.doi: 10.7506/spkx1002-6630-201201015

• Basic Research • Previous Articles Next Articles

Purification and Identification of Proline-rich Polypeptides from Bovine Colostrum

YU Fang1，LI De-long1，ZHANG Bo1，GAO Yan-ling1，ZHANG Shao-hui1,2,*

1. School of Agriculture and Biology, Shanghai JiaoTong University, Shanghai 200240, China；
2. Luh Bor. S. Research Center for Food Safety, Shanghai JiaoTong University, Shanghai 200240, China

Received:2011-01-24 Revised:2012-01-09 Online:2012-01-15 Published:2012-01-12
Contact: Shao-Hui ZHANG E-mail:yuflili@163.com

Abstract

Abstract:

Bovine colostrum was extracted to obtain bioactive proline-rich polypeptides (PRP). The molecular weight and amino acid composition of PRP were analyzed by means of SDS-PAGE and an automated amino acid analyzer, respectively. Reversed-phase high performance liquid chromatography (RP-HPLC) was employed to purify PRP. The results showed that the proline content in PRP was as high as 20%. The molecular weights of principal components were in the range of 5－25 kD as determined by SDS-PAGE. RP-HPLC showed that major spectral peaks were eluted by 37%－45% acetonitrile. As a result, PRP with a purity of 96.7% was obtained.

Key words: proline-rich polypeptide, molecular weight, amino acid composition, reversed-phase chromatography

CLC Number:

TS252.1

YU Fang，LI De-long，ZHANG Bo，GAO Yan-ling，ZHANG Shao-hui. Purification and Identification of Proline-rich Polypeptides from Bovine Colostrum[J]. FOOD SCIENCE, 2012, 33(1): 77-80.

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Purification and Identification of Proline-rich Polypeptides from Bovine Colostrum

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