Optimization of Preparation Process for Cod Skin Collagen Peptide-Iron (Ⅱ) Chelate via Response Surface Methodology

doi:10.7506/spkx1002-6630-201202011

FOOD SCIENCE ›› 2012, Vol. 33 ›› Issue (2): 48-52.doi: 10.7506/spkx1002-6630-201202011

• Processing Technology • Previous Articles Next Articles

Optimization of Preparation Process for Cod Skin Collagen Peptide-Iron (Ⅱ) Chelate via Response Surface Methodology

CAI Bing-na，CHEN Xin，PAN Jian-yu，DENG Wan-hua，WAN Peng，CHEN De-ke，SUN Hui-li

1. Key Laboratory of Marine Bio-resoures Sustainable Utilization, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China；2. Graduate University of Chinese Academy of Sciences, Beijing 100049, China； 3. Department of Chemistry and Chemical Engineering, Foshan University, Foshan 528000, China

Online:2012-01-25 Published:2012-01-16

Abstract

Abstract: The optimal chelating conditions for cod skin collagen peptide and ferrous chloride were investigated by response surface methodology (RSM) to explore the effects of pH (X1), collagen peptide-ferrous chloride ratio (X2) and collagen peptide concentration (X3) on chelating efficiency. The optimal chelating conditions were collagen peptide-ferrous chloride ratio of 4:1, collagen peptide concentration of 3.5% and pH 7.0. Under the optimal chelating conditions, the predicted and experimental yields of the peptide-iron (Ⅱ) chelate were 37.46% and 37.31%, respectively. The infrared spectral analysis revealed that the ferrous ions were chelated with NH2+ and COO－ groups, suggesting the formation of a new kind of peptide-iron (Ⅱ) chelate.

Key words: cod skin collagen peptide, response surface methodology, peptide-iron (Ⅱ) chelate

CLC Number:

S931.9

CAI Bing-na, CHEN Xin, PAN Jian-yu, DENG Wan-hua, WAN Peng, CHEN De-ke, SUN Hui-li. Optimization of Preparation Process for Cod Skin Collagen Peptide-Iron (Ⅱ) Chelate via Response Surface Methodology[J]. FOOD SCIENCE, 2012, 33(2): 48-52.

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Optimization of Preparation Process for Cod Skin Collagen Peptide-Iron (Ⅱ) Chelate via Response Surface Methodology

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