FOOD SCIENCE ›› 2012, Vol. 33 ›› Issue (3): 101-104.doi: 10.7506/spkx1002-6630-201203022

• Bioengineering • Previous Articles     Next Articles

Effect of Oligo-chitosan on the Growth of Intestinal Microorganisms as Determined by MTT Assay

LI Na，XIAO Kai-jun，PANG Hao，WANG Zhao-mei   

  1. 1. College of Light Industry and Food Science, South China University of Technology, Guangzhou 510640, China； 2. Key Laboratory of Cellulose and Lignocellulosics Chemistry, Guangzhou Institute of Chemistry, Chinese Academy of Sciences, Guangzhou 510650, China
  • Online:2012-02-15 Published:2012-02-14

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Abstract: The effect of oligo-chitosan on the growth of intestinal microorganisms was studied using MTT assays. The results indicated that oligo-chitosan could obviously accelerate the proliferation of Lactobecillus bulgaricus and Streptococcu themophilus at the concentration range of 0.1－0.8 g/100 mL, while the antibacterial activity of oligo-chitosan against Staphylococcus aureus and Salmonella was strengthened with increasing oligo-chitosan concentration. The oligo-chitosan with molecular weight of 1 kD revealed more obvious impact on the growth of intestinal microorganisms than the oligo-chitosan with molecular weight of 3 kD.

Key words: MTT assay, oligo-chitosan, intestinal microorganisms

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