FOOD SCIENCE ›› 2012, Vol. 33 ›› Issue (3): 203-206.doi: 10.7506/spkx1002-6630-201203042

• Bioengineering • Previous Articles     Next Articles

Molecular Typing Evaluation of Salmonella in Diversilab System

XU Long-yan,YUAN Mu-yun,LIN Wen-li,ZHANG Wang,JIAO Hong   

  1. Food Laboratory of Technology Center, Guangdong Entry-Exit Inspection and Quarantine Bureau, Guangzhou 510623, China
  • Online:2012-02-15 Published:2012-02-14

Abstract: Objective: To evaluate the typing performance of DiversiLab system (DVL) for Salmonella. Method: A DVL system, which is based on rep-PCR, was used to type 44 Salmonella isolates from exported and imported foods and compared with pulsed-field gel electrophoresis (PFGE). The Salmonella isolates were typed by rep-PCR into 22 groups. The same serotypes of Salmonella isolates from different countries and foods were distributed in the same group with a high similarity. The PFGE results obtained for Salmonella isolates assigned to the same group using rep-PCR indicated a low similarity among all other isolates except No. 2 and No. 15. Conclusion: DVL shows better typing ability but lower resolution for Salmonella than PFGE.

Key words: Salmonella, DiversiLab system, rep-PCR, PFGE, molecular typing

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