Determination of Hydroxyproline in Fishery Products by Pre-Column Derivatization-High Performance Liquid Chromatography

doi:10.7506/spkx1002-6630-201208045

FOOD SCIENCE ›› 2012, Vol. 33 ›› Issue (8): 207-210.doi: 10.7506/spkx1002-6630-201208045

• Analysis & Detection • Previous Articles Next Articles

Determination of Hydroxyproline in Fishery Products by Pre-Column Derivatization-High Performance Liquid Chromatography

HUANG Hui1，GONG Xiang-hong2,3，LIU Hui-hui2,3，XU Ying-jiang2,3，LI Jia-wei4，ZHANG Xiu-zhen2,3,*

(1. College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China； 2. Marine Fisheries Research Institute of Shandong Province, Yantai 264006, China； 3. Shandong Provincial Key Laboratory of Restoration for Marine Ecology, Yantai 264006, China； 4. Yantai Shanshui Seafood Co. Ltd., Yantai 264006, China)

Online:2012-04-25 Published:2012-03-31

Abstract

Abstract: A high performance liquid chromatographic method was established for the determination of hydroxyproline in fishery products with pre-column derivatization. Samples were hydrolyzed with hydrochloric acid before pre-column derivatization with dansyl chloride. The analyze was separated using a C18 column and detected using a UV detector. Good separation was achieved for hydroxyproline and other amino acids. The linear range was 0.5－20 μg/mL (r ＞ 0.9999). The detection limit was 0.5 μg/mL. The average spike recovery rates were 80.2%－103% with a RSD of 3.2%－5.6%. The method was sensitive, accurate and suitable for the determination of hydroxyproline in fishery products.

Key words: hydroxyproline, dansyl chloride, pre-column derivatization, HPLC, fishery products

CLC Number:

O657.72

HUANGHui，GONGXiang-hong，LIUHui-hui，XUYing-jiang，LIJia-wei，ZHANGXiu-zhen,. Determination of Hydroxyproline in Fishery Products by Pre-Column Derivatization-High Performance Liquid Chromatography[J]. FOOD SCIENCE, 2012, 33(8): 207-210.

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Determination of Hydroxyproline in Fishery Products by Pre-Column Derivatization-High Performance Liquid Chromatography

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