FOOD SCIENCE ›› 2012, Vol. 33 ›› Issue (9): 127-130.doi: 10.7506/spkx1002-6630-201209027

• Bioengineering • Previous Articles     Next Articles

Optimization of Fermentation Medium Based on Chinese Medicinal Herbs for Mycelial Growth of Grifola frondosa by Response Surface Methodology

HU Xiao-jing1,XIE Ying-li1,YE Qun1,WU Tian-xiang2,YANG Hai-long1,*   

  1. (1. School of Life and Environmental Sciences, Wenzhou University, Wenzhou 325035, China; 2. School of Life Science, Guizhou University, Guiyang 550025, China)
  • Online:2012-05-15 Published:2012-05-07

Abstract: In order to maximize the mycelial growth of Grifola frondosa in submerged culture, the optimal carbon and nitrogen sources were screened out of 6 Chinese edible and medicinal herbs including Radix Dioscoreae oppositae, Semen Nelumbo, black rice, Radix Codonopsis pilosulae, Semen Coicis lachryma jobi, and Fructus lycii and the medium composition was optimized using Box-Behnken experimental design coupled with response surface analysis. Radix Codonopsis pilosulae and Fructus lycii were suitable for use as sole carbon sources and Semen Nelumbo was the most suitable nitrogen source. The optimized medium composition was composed of 23.5 g/L Fructus lycii, Semen Nelumbo 4.3 g/L and glucose 28.8 g/L, resulting in a mycelial biomass of 16.937 g/L.

Key words: Chinese edible and medicinal herbs, Grifola frondosa, submerged fermentation, response surface methodology

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