Screening of Strains with High Antibacterial Activity from Edible and Medicinal Fungi and Optimization of Culture Medium

doi:10.7506/spkx1002-6630-201209029

Abstract

Abstract: The antibacterial activities of 24 edible or medicinal fungus strains belonging to 4 genera against 11 indicator bacteria were studied. Three edible fungi with obvious antibacterial activity and 6 indicator bacterial strains were preliminarily screened. Secondary screening was carried out to pick out strain H2 and three indicator bacteria including Bacillus subtilis, Salmonella typhi, and Bacillus cereus for further research into the optimization of culture medium for strain H2 using response surface methodology based on inhibitory zone radii against the indicator bacteria. The results showed that the optimal culture medium for strain H2 was composed of 4.09% maltose, 1.12% peotone, 0.21% KH2PO4, and 0.11% MgSO4 ·7H2O. The inhibitory zone radii of the strain against B. subtilis, S. typhi and B. cereus were 3.513, 4.610 mm and 8.800 mm, respectively after culture in the medium.

Key words: edible and medicinal fungi, antibacterial activity, screening, culture medium, response surface methodology

CLC Number:

XIELi-yuan，LEIWan-yong，DUSi-yun，GANBing-cheng，PENGWei-hong. Screening of Strains with High Antibacterial Activity from Edible and Medicinal Fungi and Optimization of Culture Medium[J]. FOOD SCIENCE, 2012, 33(9): 138-143.

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Screening of Strains with High Antibacterial Activity from Edible and Medicinal Fungi and Optimization of Culture Medium

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