Selective Enrichment and Real time PCR Detection of Thermal Injured Salmonella in Food

doi:10.7506/spkx1002-6630-201210046

Abstract

Abstract: A selective enrichment broth, SEL based real time PCR assay was established after treating a sublethal injured Salmonella cells by heat-shock. The results showed that 1－2 CFU of injured Salmonella cells in 5 mL SEL could be fully recovered and enriched to the level of 109 CFU/mL after a 20 h enrichment step even without the nonselective recovery process. The amplification efficiency of real time PCR was 95.41% and the detection limit was 4 CFU/reaction for Salmonella genomic DNA. The detection limit in artificially contaminated food sample was 3 CFU/10 g ground beef, and showed a high coincidence to the detection result by traditional culture-based assay. The overall recovery, selective enrichment and detection procedure of thermal injured Salmonella could be accomplished in 24 h, which showed a high potential of application in the effective detection of Salmonella in food.

Key words: thermal injury, Salmonella, selective enrichment, real time polymerase chain reaction (PCR), detection

CLC Number:

TS254.1

SUOBiao，TENGYao-hui，SHIXian-ming，AIZhi-lu. Selective Enrichment and Real time PCR Detection of Thermal Injured Salmonella in Food[J]. FOOD SCIENCE, 2012, 33(10): 223-227.

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Selective Enrichment and Real time PCR Detection of Thermal Injured Salmonella in Food

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