Amino Acid Compositions of Silkworm Pupa Protein and Its Hydrolysates

doi:10.7506/spkx1002-6630-201212046

Abstract

Abstract: The amino acid compositions of silkworm pupa protein and its hydrolysates prepared with neutral protease were determined by reversed-phase high performance liquid chromatography (RP-HPLC) with precolumn derivatization. Meanwhile, their ACE inhibitory activity was also tested. The results indicated that the content of hydrophobic amino acids in the hydrolysate ultrafiltration fraction SN2 (＜ 10 kD) was 49.15% compared to 40.55% for the hydrolysate ultrafiltration fraction SN1 (＞10 kD) and 45.21% for silkworm pupa protein. The contents of proline and aromatic amino acids (Trp, Phe and Tyr), which had great effect on ACE inhibitory activity, were 7.42% and 15.81%, respectively. Moreover, the ACE inhibitory rate of SN2 was 47.6%, which was remarkably higher than that of SN1 and silkworm pupa protein, suggesting that the change trends of hydrophobic amino acid composition and ACE inhibitory activity are the same and that the ACE inhibitory activity is mostly concentrated on SN2.

Key words: silkworm pupa, hydrolysate, amino acid, high performance liquid chromatography (HPLC), ACE inhibitory activity

CLC Number:

Q566.3

LU..Wen-jun，ZHAO Zhong-xing，LIAO Dan-kui，SUN Jian-hua，HUANG Ke-lin，SUN Guo-song，WU Zhi-hong，TONG Zhang-fa. Amino Acid Compositions of Silkworm Pupa Protein and Its Hydrolysates[J]. FOOD SCIENCE, 2012, 33(12): 228-232.

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Amino Acid Compositions of Silkworm Pupa Protein and Its Hydrolysates

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