Supercritical Fluid Chromatographic Separation of Eicosapentaenoic Acid Ethyl Ester and Docosahexaenoic Acid Ethyl Ester from Fish Oil

doi:10.7506/spkx1002-6630-201224017

Abstract

Abstract:

A supercritical fluid chromatographic method to separate eicosapentaenoic acid ethyl ester (EPA-EE) and docosahexaenoic acid ethyl ester (DHA-EE) from fish oil was established. Hedera ODS-BP was found to be the most effective column for separating EPA-EE and DHA-EE from fish oil as demonstrated by a comparison with Luna 5u Silica, Hypersil ODS2, ZORBAX SB-C8, and Hypersil C8. The effects of mobile phase modifier type, methanol percentage in mobile phase, back pressure and column temperature for the separation of both esters by Hedera ODS-BP on capacity factor, diastereoselectivity and resolution were investigated. The optimum conditions forthese factors were found to be separation on Hedera ODS-BP column at a column temperature of 40 ℃ an a back pressure of 100 bar using a mobile phase containing 10% methanol as a modifier at a total flow rate of 2 mL/min. Under these conditions, baseline separation of EPA-EE and DHA-EE was achieved. The developed method was simple, rapid, highly reproducible and suitable for the separation of EPA-EE and DHA-EE from fish oil.

Key words: supercritical fluid chromatography (SFC), eicosapentaenoic acid ethyl ester (EPA-EE), docosahexaenoic acid ethyl ester (DHA-EE), separation

CLC Number:

O657.74

CHEN Wei-zhu1,2，HONG Zhuan 1,*，ZHAO Min1，ZHANG Yi-ping1，XIE Quan-ling 1，YI Rui-zao 1. Supercritical Fluid Chromatographic Separation of Eicosapentaenoic Acid Ethyl Ester and Docosahexaenoic Acid Ethyl Ester from Fish Oil[J]. FOOD SCIENCE, 2012, 33(24): 82-85.

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Supercritical Fluid Chromatographic Separation of Eicosapentaenoic Acid Ethyl Ester and Docosahexaenoic Acid Ethyl Ester from Fish Oil

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