Spectrophotometric Determination of Trace Chromium in Dairy Products Using Nuclear Fast Red

doi:10.7506/spkx1002-6630-201224060

Abstract

Abstract: A new spectrophotometric method for the simultaneous determination of chromium (Ⅲ) and chromium (Ⅵ) in dairy products was proposed. This method was based on the oxidative decoloration of nuclear fast red by chromium (Ⅵ) in hydrochloric acid solution, which is linearly related to chromium (Ⅵ) concentration in a certain range. The oxidization of chromium (Ⅲ) to chromium (Ⅵ) by hydrogen peroxide in sodium hydroxide solution allows the determination of total chromium and the consequent calculation of chromium (Ⅲ). The wavelength of maximum absorbance was 560 nm with an apparent molar absorption coefficient of 2.2 × 104 L/(mol•cm). The absorbance obeyed Beer-Lambert law over the range of 0.2–1.6 µg/mL for chromium (Ⅵ). The proposed method was applied to determine chromium (Ⅲ) and chromium (Ⅵ) in several kinds of dairy products and the results were in good agreement with those determined by 1,5-diphenylcarbazide spectrophotometry.

Key words: nuclear fast red, chromium (Ⅲ), chromium (Ⅵ), spectrophotometry, dairy products

CLC Number:

O657.32

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